DnaA5 protein is thermolabile in initiation of replication from the chromosomal origin of Escherichia coli
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منابع مشابه
Purified dnaA protein in initiation of replication at the Escherichia coli chromosomal origin of replication.
Soluble protein fractions from Escherichia coli dnaA+ cells but not dnaA temperature-sensitive cells replicate plasmids containing the E. coli chromosomal origin of replication (oriC). Complementation of these mutant fractions provided an assay for dnaA protein activity in initiation of replication at oriC. From a strain (constructed in vitro) that overproduces the dnaA protein more than 200-fo...
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Highly purified preparations of dnaA46 protein have permitted its biochemical characterization in comparison with the activities of wild type dnaA protein. We have determined that dnaA46 protein was reduced in its ability to bind to DNA fragments containing oriC. This mutant protein was also defective in binding ATP and was inactive for replication of oriC-containing plasmids in purified enzyme...
متن کاملControlled initiation of chromosomal replication in Escherichia coli requires functional Hda protein.
Regulatory inactivation of DnaA helps ensure that the Escherichia coli chromosome is replicated only once per cell cycle, through accelerated hydrolysis of active replication initiator ATP-DnaA to inactive ADP-DnaA. Analysis of deltahda strains revealed that the regulatory inactivation of DnaA component Hda is necessary for maintaining controlled initiation but not for cell growth or viability.
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15 صفحه اولThe DnaA Protein Is Not the Limiting Factor for Initiation of Replication in Escherichia coli
The bacterial replication cycle is driven by the DnaA protein which cycles between the active ATP-bound form and the inactive ADP-bound form. It has been suggested that DnaA also is the main controller of initiation frequency. Initiation is thought to occur when enough ATP-DnaA has accumulated. In this work we have performed cell cycle analysis of cells that contain a surplus of ATP-DnaA and as...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1993
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)38628-4